This document, developed by the World Health Organization's Tobacco Laboratory Network (TobLabNet), provides a standardized operating procedure for determining the moisture content in smokeless tobacco products. It addresses the need for consistent measurement methods to evaluate tobacco product stability and integrity. The procedure involves a specific oven-drying method to measure volatile components, crucial for assessing nicotine release. Originating from a request by the WHO Framework Convention on Tobacco Control, it aims to support public health research and regulation by providing validated methodologies. The intended audience includes health researchers and regulatory bodies focused on tobacco control.