This laboratory manual, published by the World Health Organization in 2024, offers a comprehensive guide to the diagnosis and management of yellow fever. It covers the structure, biology, and transmission of the yellow fever virus, as well as the human immune response to infection. The manual also addresses epidemiological aspects, vaccination strategies, and global surveillance efforts. It provides detailed methodologies for laboratory testing, including the collection and handling of clinical samples, and outlines algorithms for screening and confirmation of yellow fever infections. Aimed at healthcare professionals and laboratory technicians, this manual is part of the broader EYE strategy, focusing on strengthening laboratory capacities in Africa, Latin America, and the Caribbean. The book is intended as a resource for enhancing laboratory practices and ensuring accurate diagnosis and reporting of yellow fever cases.