Tropane alkaloids are a group of toxic compounds occurring in several plant families of the Solanaceae family-like Datura stramonium, that can contaminate staples like cereals and grains. The Joint FAO/WHO Expert Meeting on tropane alkaloids carried out a risk assessment of these contaminants in certain food products distributed by the United Nations World Food Programme (WFP) and provided guidance for the development of appropriate operational limits. This document builds on the deliberations from the expert meeting and provides specific guidance on the physical contamination of D. stramonium seeds in different agricultural products, namely wheat, soybean, and maize. This guidance document can be used in conjunction with the 'Report of the Joint FAO/WHO Expert Meeting on Tropane Alkaloids' to prevent contamination of cereals and grains with D. stramonium seeds, thereby also averting human poisoning outbreaks.