Research Paper (postgraduate) from the year 2012 in the subject Medicine - Diagnostics, ( Atlantic International University ) (Atlantic International University, Honolulu, Hawaii), course: Health Sciences, language: English, abstract: A total of 256 sputum samples, were stained using Ziehl Neelsen method, in Medical Laboratory section of Hospital of Porto Novo, between 3rd of July, 2008 to 30th of April, 2012. A total of 16 (6.25%) of the samples were positive for mycobacterium tuberculosis, while 240 (93.75%) were negative. Males (n=131) showed higher infectivity 14 (10.68%) than females (n=125) that recorded 2 (1.6%).This research concluded that, free and appropriate treatment for all the infected persons, improving laboratory diagnostic facilities, and further research, to include, cultural and antigen detection assay were the areas that needed immediate attention.