While automated trucking developers have established regular commercial shipments, operations and testing remain limited largely to limited-access highways like interstates. This infrastructure provides a platform or operating environment that is highly structured, with generally good road conditions and visible lane markings. To date, these deployments have not included routine movements from hub to hub, whether on or off these limited-access facilities. Benefits such as safety, fuel efficiency, staffing for long-haul trips, and a strengthened supply chain turn enable broader deployment which can enable movement from one transportation system to another. Infrastructure Enablers and Automated Vehicles: Trucking focuses on unresolved issues between the automated vehicle industry and infrastructure owners and operators that stand in the way of using infrastructure—both physical and digital—to extend use cases for automated trucking to more operational design domains (ODDs). The report also examines opportunities and recommendations related the integration of automated trucking across transportation networks and the supply chain. The topics include road conditions and lane marking visibility, work zone navigation, transfer hubs, and facility design, as well as connected and electric charging infrastructure. Click here to access The Mobility Frontier: Accelerating Infrastructure Readiness for Autonomy Click here to access the full SAE EDGETM Research Report portfolio. https://doi.org/10.4271/EPR2022017